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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
15/10/2015 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA JÚNIOR, J. V. J.; ARENHART, S.; SANTOS, H. F. dos; ALMEIDA-QUEIROZ, S. R.; SILVA, A. N. M. R.; TREVISOL, I. M.; BERTANI, G. R.; GIL, L. H. V. G. |
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Afiliação: |
JOSÉ VALTER JOAQUIM SILVA JÚNIOR, FIOCRUZ; SANDRA ARENHART, FIOCRUZ; HELTON FERNANDES DOS SANTOS, UFRGS/ICBS; SABRINA RIBEIRO DE ALMEIDA-QUEIROZ, FIOCRUZ; ANDRÉA NAZARÉ MONTEIRO RANGEL DA SILVA, FIOCRUZ; IARA MARIA TREVISOL, CNPSA; GIOVANI ROTA, UFPE/LIKA; LAURA HELENA VEGA GONZALES GIL, FIOCRUZ. |
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Título: |
Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555-1563, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world?s first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development. |
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Palavras-Chave: |
IBVD; Virologia molecular. |
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Thesagro: |
Doença de Gumboro; Frango; Genética molecular. |
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Thesaurus Nal: |
Avibirnavirus; Chickens; Infectious bursal disease virus; Molecular genetics; Vertebrate viruses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131119/1/final7824.pdf
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Marc: |
LEADER 02128naa a2200325 a 4500
001 2026478
005 2018-01-26
008 2014 bl uuuu u00u1 u #d
100 1 $aSILVA JÚNIOR, J. V. J.
245 $aEfficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast.$h[electronic resource]
260 $c2014
520 $aThe Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world?s first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development.
650 $aAvibirnavirus
650 $aChickens
650 $aInfectious bursal disease virus
650 $aMolecular genetics
650 $aVertebrate viruses
650 $aDoença de Gumboro
650 $aFrango
650 $aGenética molecular
653 $aIBVD
653 $aVirologia molecular
700 1 $aARENHART, S.
700 1 $aSANTOS, H. F. dos
700 1 $aALMEIDA-QUEIROZ, S. R.
700 1 $aSILVA, A. N. M. R.
700 1 $aTREVISOL, I. M.
700 1 $aBERTANI, G. R.
700 1 $aGIL, L. H. V. G.
773 $tBrazilian Journal of Microbiology$gv. 45, n. 4, p. 1555-1563, 2014.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 5 | |
| 1. |  | SANTOS, H. F. dos; FLORES, M. L.; LARA, V. M.; TREVISOL, I. M.; LOVATO, L. T. Anticorpos contra vírus de galinha em cracídeos Ciência Rural, Santa Maria, v.39, n.7, p.2225-2228, out, 2009. Disponível em: . Acesso em: 05 abr. 2010. Projeto/Plano de Ação: 16.00.30004-00| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 2. | | SANTOS, H. F. dos; LOVATTO, L. T.; FLÔRES, M. L.; TREVISOL, I. M.; MAZZUTTI, K. C.; PAN, K. A. Anticorpos contra vírus em galinhas de terreiro do estado do Rio Grande do Sul, Brasil. Ciência Rural, v.38, n.7, p.1932-1937, 2008. Projeto/Plano de Ação: 16.00.30.004-00.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | VARELA, A. P. M.; SANTOS, H. F. dos; CIBULSKI, S. P.; SCHEFFER, C. M.; SCHMIDT, C.; LIMA, F. E. S.; SILVA, A. D.; ESTEVES, P. A.; FRANCO, A. C.; ROEHE, P. M. Chicken anemia virus and avian gyrovirus 2 as contaminants in poultry vaccines. Biologicals, London, v. 42, n. 6., p. 346–350, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | SILVA JÚNIOR, J. V. J.; ARENHART, S.; SANTOS, H. F. dos; ALMEIDA-QUEIROZ, S. R.; SILVA, A. N. M. R.; TREVISOL, I. M.; BERTANI, G. R.; GIL, L. H. V. G. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555-1563, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
|  |
| 5. | | SANTOS, H. F. dos; KNAK, M. B.; CASTRO, M. L. de; SLONGO, J.; RITTERBUSCH, G. A.; KLEIN, T. A. P.; ESTEVES, P. A.; SILVA, A. D.; TREVISOL, I. M. Variants of the recently discovered avian gyrovirus 2 area detected in Southern Brazil and The Netherlands Veterinary Microbiology, v. 155, n. 2-4, p. 230-236, 2012. Projeto/Plano de Ação: 02.09.13000-04.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 5 | |
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